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| Avellar-Costa,Pedro; Santos,Danielle Cabral dos; Lange,Carla Christine; Laport,Marinella Silva; Brito,Maria Aparecida Vasconcelos Paiva; Rossi,Ciro César; Giambiagi-deMarval,Marcia. |
| ABSTRACT: We compared the potential of routine techniques used for the identification of Staphylococcus species, aiming to evaluate their accuracy in the detection of 43 Staphylococcus chromogenes strains isolated from bovine mastitis that, despite being a coagulase-negative species, are able to clot plasma. These strains could be mistakenly suspected to be S. aureus and lead to an unappropriated treatment of the disease. MALDI-TOF, PCR-RFLP of the chaperonine gene groEL, and sequencing of the 16S rRNA and elongation factor Tu gene tuf were employed. Results from the four methods were coincident for only half of the strains because of the low accuracy of the groEL PCR-RFLP (51.2% accuracy). Even though all the sequencing results were identical, the high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus chromogenes; Mastitis; MALDI-TOF; Tuf; GroEL. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600452 |
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| Laport,Marinella Silva; Pontes,Paula Veronesi Marinho; Santos,Daniela Silva dos; Santos-Gandelman,Juliana de Fátima; Muricy,Guilherme; Bauwens,Mathieu; Giambiagi-deMarval,Marcia; George,Isabelle. |
| ABSTRACT Although antibiotic-resistant pathogens pose a significant threat to human health, the environmental reservoirs of the resistance determinants are still poorly understood. This study reports the detection of resistance genes (ermB, mecA, mupA, qnrA, qnrB and tetL) to antibiotics among certain culturable and unculturable bacteria associated with the marine sponge Petromica citrina. The antimicrobial activities elicited by P. citrina and its associated bacteria are also described. The results indicate that the marine environment could play an important role in the development of antibiotic resistance and the dissemination of resistance genes among bacteria. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbes; Natural hotspots; Porifera; Resistance; Rio de Janeiro. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300617 |
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| Rossi,Ciro César; Pereira,Monalessa Fábia; Giambiagi-deMarval,Marcia. |
| Abstract The increasing threat of antimicrobial resistance has shed light on the interconnection between humans, animals, the environment, and their roles in the exchange and spreading of resistance genes. In this review, we present evidences that show that Staphylococcus species, usually referred to as harmless or opportunistic pathogens, represent a threat to human and animal health for acting as reservoirs of antimicrobial resistance genes. The capacity of genetic exchange between isolates of different sources and species of the Staphylococcus genus is discussed with emphasis on mobile genetic elements, the contribution of biofilm formation, and evidences obtained either experimentally or through genome analyses. We also discuss the involvement of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Horizontal gene transfer; Antimicrobial resistance; Coagulase negative staphylococcus; Biofilm; CRISPR; One Health. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000300303 |
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